Integrating Chemical Tools to Interrogate Protein Methylation

Title:

Integrating Chemical Tools to Interrogate Protein Methylation

Schedule:

Date: 24^th May, 2024 (Friday)

Time: 5 - 6 pm (HKT)

Venue: Lecture Theatre P2, Chong Yuet Ming Physics Building

Speaker:

Prof. Minkui Luo

Chemical Biology Program, Memorial Sloan Kettering Cancer Center

New York

Abstract:

Epigenetic biology comprises a collection of inherited cellular cues that enable diverse cell fates to be determined from a single genome. The ensemble of epigenetic events maintains normal cellular functions and its dysregulation is frequently implicated in disease. Among the critical epigenetic events is protein methylation, which is deposited by ~100 methyltransferases, removed by ~30 demethylases, and recognized by hundreds of effectors. Elucidating diverse epigenetic functions of protein methylation is pivotal toward elucidating their roles in normal physiology and disease states. The Luo lab has long-term interest in applying interdisciplinary chemical biology principles to annotate diverse protein methylation events. We have developed the Bioorthogonal Profiling of Protein Methylation (BPPM) with cofactor analogues for chemical labeling of methylome in vitro and within live cells. We also developed the complementary technology to annotate the upstream methyltransferase activities and downstream effectors of designated methylation events. This presentation will highlight these technologies and their collective application to annotating the complex methylome network in the context of tumor malignancy and ESC pluripotency.

- ALL ARE WELCOME -